03/25/2026
For our final CaBSSem of the year (Friday, Mar 27), Richard Brown will be joining us from Dalhousie University.
Presenter: Richard Brown (Dalhousie University)
Title: Developing a one day test for studying short, long, and very-long term olfactory memory in mice
Abstract:
Schellinck et al (2001) developed a simple and reliable test of olfactory learning and memory in mice using a Pavlovian conditioned odour preference task in which CD1 mice learned to discriminate between two odours after two daily 10-min trials with the CS+ odour (sugar reward) and two trials with the CS− odour (no sugar) over 4 days. Mice remembered the CS+ for up to 60 days after training in this study and, in other studies, male and female C57BL/6J and DBA/2J mice could remember the odour discrimination for up to 9 months after training. Mice up to 24 months of age can learn and remember the conditioned odour preferences (Wong & Brown 2007). Likewise, the 5×FAD mouse model of Alzheimer disease showed no odour memory deficits in this task (O’Leary et al., 2020).
In the present series of studies we developed a 1-day training procedure in which male and female mice received eight CS+ and eight CS− odour presentations on day 1 and were then tested for memory 24 h, 7 days and 30 days later. Mice (C57BL/6J × SJL/J and C57BL/6J) given the 1-day training showed a conditioned odour preference on 24 h, and 7-day memory tests with no s*x differences. After 30 days the odour preference was not significant. Mice (C57BL/6J) given four CS+ and four CS− trials showed no significant odour preference on 24 h and 7-day memory tests. The results suggest that different 1-day training paradigms result in different memory strength. Such a 1-day appetitive training test could be valuable for studying the neurobiological bases of short-, long-, and very long-term memory (Brown et al., 2023; 2024).
Learning and memory involves a two-step process: first there is the Pavlovian conditioned association between the CS+ odour and sugar reward [and the conditioned association between the CS- odour and no reward]. Second, there is the instrumental motor response in searching for, finding and eating the sugar associated with the CS+ odour. Since the mouse learns a “rule” during the Pavlovian conditioning (Autoshaping), it should be able to utilize that rule in transfer of training tasks, such as learning a T-maze for odour reward. We have been examining the strength of memory using transfer of training tasks at 7 and 30 days after conditioning. The results will surprise you and I will show videos of mice during training, memory testing and transfer of training tasks to show you how we use our Pavlovian conditioned odour preference task to study the mind of the mouse in the same way the Pavlov used the conditioned salivary response to study the mind of the dog.
The talk will be in person in the Psychology Reading room, COR A228, from 3:00 – 4:30.
Hope you can join!