Exeter IGEM 2020

Exeter IGEM 2020 Welcome to the University of Exeter's 2020 iGEM team page.

Our project presentation is now live!This presentation contains a full breakdown of our project: Detailing why we chose ...
30/10/2020

Our project presentation is now live!

This presentation contains a full breakdown of our project: Detailing why we chose corals to how we would plan to design and build our 3D printer.

Presentation Video for CalcifEXE, Exeter University's iGEM project for 2020. We are genetically engineering bacteria in order to produce calcium carbonate, w...

๐ŸŽ‰...and our wiki is finished!๐ŸŽ‰If you are keen to check it out it can be found at:
28/10/2020

๐ŸŽ‰...and our wiki is finished!๐ŸŽ‰

If you are keen to check it out it can be found at:

We are engineering Bacillus subtilis to enhance its ability to produce precipitated calcium carbonate (PCC) in different crystal structures.

โ€œMy wish is that this will provide a positive message to the young girls who would like to follow the path of science, a...
08/10/2020

โ€œMy wish is that this will provide a positive message to the young girls who would like to follow the path of science, and to show them that women in science can also have an impact through the research that they are performing.โ€ - 2020 Chemistry Laureate Emmanuelle Charpentier.

The Exeter iGEM Team would love to extend our congratulations to Professors Charpentier and Doudna for receiving the Nobel Prize for Chemistry 2020. Their work with CRISPR/Cas9 pushed synthetic biology into a new frontier!

We are also thrilled to see (for the first time in history) two women jointly winning this coveted prize, bringing the total number of women to have ever received it up to 7.

Alternative Text:

The 2020 Nobel Prize in Chemistry is awarded to Emmanuelle Charpentierย and Jennifer A. Doudnaย โ€œfor the development of a method for genome editingโ€.
Since Charpentier and Doudna discovered the CRISPR/Cas9 genetic scissors in 2012 their use has exploded. The genetic scissors have taken the life sciences into a new epoch and, in many ways, are bringing the greatest benefit to humankind.

Thank you team UCL for providing our team with some useful HTML and CSS tips for our wiki! You have been really helpful!
08/10/2020

Thank you team UCL for providing our team with some useful HTML and CSS tips for our wiki! You have been really helpful!

In the US, a significant proportion of the food consumed is safely genetically modified (for example 92% of corn eaten i...
06/10/2020

In the US, a significant proportion of the food consumed is safely genetically modified (for example 92% of corn eaten in the US is genetically modified either by adding genes from Bacillus thuringiensis (making it resistant to insect attack) or from Agrobacterium (resistant to the weedkiller Roundup)). In the UK, no GM food is consumed by law and thus, concerns about GM food in this country are generally unprompted. Hopefully you believe these recent infographics have answered all the questions you raised in the survey about the safety of our project!

According to WHO, there are five major concerns surrounding GMOs and the environment:1) The capability of the GMO to esc...
04/10/2020

According to WHO, there are five major concerns surrounding GMOs and the environment:
1) The capability of the GMO to escape and introduce engineered genes into wild populations
2) Susceptibility of (non-target) organisms to the gene product
3) The stability of the gene
4) Impacts on biodiversity
5) Increased use of chemicals (especially in the case of herbicides and GM plants)
If GMOs are introduced into the environment, they can affect biodiversity (e.g. more dominant new species can outcompete existing species). Although these same effects often also arise from the introduction of non-GM species, these potential effects are always considered, even if the chance a GM species can escape are incredibly slim.
As a team, we are following strict iGEM guidelines, including preventing GMO release into the environment by wearing safety gear in the lab to prevent contamination, using antibiotic resistance genes where necessary, and disposing of contaminated equipment in autoclave bags to ensure the GMO never leaves the lab. These guidelines make sure GM organisms never make it out of the laboratory during our research.
Alongside this, we are planning to test the efficacy of several different methods of removing GM bacteria from our calcium carbonate product before it is introduced to the natural environment. These potential methods include exposure to UV light, the introduction of a genetic kill switch, or treatment with antibacterials. We will be ensuring that the chosen method kills off one hundred percent of our bacteria before we even consider implementing it into the manufacturing process.

We hope this addresses any concerns you might have about our project and the safety concerns surrounding our GM bacteria getting into the environment ๐Ÿ˜Š

Great to catch up with UCL this morning! Thank you guys for speeking with us.
02/10/2020

Great to catch up with UCL this morning! Thank you guys for speeking with us.

We have a YouTube channel!!! We currently have 3 videos published for you to watch and enjoy with many more on their way...
26/09/2020

We have a YouTube channel!!! We currently have 3 videos published for you to watch and enjoy with many more on their way! To find it please check on the link in our bio (https://www.youtube.com/channel/UC8K5fDp1fMhx6WURwiXM_xQ) or search 'Exeter iGEM 2020' on YouTube! It would also mean a lot to all members of the team if you could like and subscribe ๐Ÿ˜Š

CalcifEXE, a low carbon 3D bioprinting technique of calcium carbonate. Watch our promo video now for more details! ๐Ÿ‘‡
24/09/2020

CalcifEXE, a low carbon 3D bioprinting technique of calcium carbonate.

Watch our promo video now for more details! ๐Ÿ‘‡

CalcifEXE, a low carbon 3D bioprinting technique of calcium carbonate. Find out more: https://2020.igem.org/Team:Exeter

H U M A N    P R A C T I C E SHuman practices is all about understanding the true impact of our project on people, socie...
18/09/2020

H U M A N P R A C T I C E S
Human practices is all about understanding the true impact of our project on people, society and the environment, then improving this impact. Here are some of the ethical issues we have considered as part of Human Practices and project development:

๐Ÿš The ethics of building a new reef ๐Ÿš
- One use of our CaCO3-producing bacteria is within the ink of a 3D printer, that could be used to print out structures for coral to grow on. Some survey respondents expressed concerns that if we grow a new reef it may lack species diversity and a proper ecosystem.
- To address this issue we have been in touch with coral ecologists at the university. From Professor Steve Simpson we learned that reef fish live within the hollow spaces in corals, so we are designing our coral backbone with a meshlike inner structure that can harbour reef life.
- New coral normally grows on top of CaCO3 skeletons produced by pre-existing coral polyps. Our printed CaCO3 structure therefore mimics natural coral habitats and should allow a healthy reef to grow with minimal human intervention.

๐Ÿงฌ The ethics of genetically engineering bacteria ๐Ÿงฌ
Genetic engineering can be used irresponsibly, and some people worry that even editing DNA for the right reasons may have unintended consequences. The rules of iGEM require us to seriously consider the effects of genetic engineering in our project.

We are adhering to these iGEM guidelines:
โœ˜ NO testing on humans (including ourselves) or tissue samples from humans
โœ˜ NO animal testing*
โœ˜ NO gene drives* (a gene drive is a genetic modification that changes the 50/50 chance of offspring inheriting a gene)
โœ˜ NO release of GMOs into the environment during project development
(Note that building a coral backbone is a theoretical application of our project; we won't have time to build and test the structure before the iGEM competition deadline. There is therefore no risk that our GM bacteria will come into contact with the ocean. We still have to consider the safety of our coral backbone in case other researchers decide to build on our work in the future - for information on how we would prevent GM bacteria release from our coral backbone, see our next infographic on Genetic Engineering and Safety)
โœ” Wearing safety gear within the lab (never outside it) to prevent contamination between the two spaces
โœ” Using antibiotic resistance genes only where necessary
โœ” Disposing of contaminated equipment in autoclave bags to ensure GMOs never leave the lab during testing phase

*these options are allowed in iGEM with special permission from the Safety Committee, however we are not using them

D E S I G N   C Y C L E     Our team used a Build-Test-Learn-Design cycle in order to optimise and improve our designs. ...
16/09/2020

D E S I G N C Y C L E

Our team used a Build-Test-Learn-Design cycle in order to optimise and improve our designs. First, our initial prototypes were synthesised following logical analysis of our chosen issues, brainstorming, outreaching to experts, research and experimentation before subjecting the designs to testing. Test data analysis informed and directed subsequent iterations of our project. Through this approach our project evolved and become what it is today! There were three design heavy elements of our project which we subjected to the Build-Test-Learn-Design cycle. These including the engineering of our Genetically modified bacteria, our printer hardware and our software.

Post description: post shows an illustration of the Test-Learn-Design-Build cycle around our Exeter iGEM logo.

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