MolE - Molecular Engineering Lab

08/01/2021

I would like to thank Francesca Susa, Tania Limongi, and Valentina Cauda for this achievement.

We have here investigated the problem of lyophilization of two liposomal drug formulations (i.e., DOPC-DOTAP and the PEGylated counterpart, and DOPC-DOTAP-DSPE-PEG). The role of various cryoprotectants was evaluated paying attention to their ability to preserve the structural integrity of liposomes.

Pisano

https://www.sciencedirect.com/science/article/pii/S0011224020306866

In the last decades, liposomes acquired a striking success in the biomedical field thanks to their biocompatibility and drug delivery ability. Many li…

22/01/2020

Congrats to all co-authors (Irene, Andrea, Chinwe, Kiran, Jackie, and Paul) for this achievement! (✍️RP)

The effect of controlled nucleation on the stability of human growth hormone (hGH) during freeze-drying has been investigated. More specifically, the vacuum-induced surface freezing technique has been compared to conventional freezing, both with and without an annealing step. Size exclusion chromatography and cell-based potency assays have been used to characterize the formation of soluble aggregates and the biological activity of hGH, respectively. The results obtained indicate that controlled nucleation has a positive effect on both cycle performance and product homogeneity because of the formation of bigger ice crystals, and characterized by a narrower dimensional distribution. For hGH stability, we observed that vacuum-induced surface freezing is not detrimental to the biological activity of the protein, or aggregate formation. Also, the effect of 2 different formulations, including trehalose or cellobiose, on protein preservation was considered for this study.

In the present work, the effect of controlled nucleation on the stability of human growth hormone (hGH) during freeze-drying has been investigated. Mo…

07/01/2020

Congrats to all co-authors: Andrea Arsiccio & Paolo Giorsello & Livio Marenco! (✍️RP) 👏👏

"Considerations on Protein Stability during Freezing and its Impact on the Freeze Drying Cycle: A Design Space Approach"

Freezing is widely used during the manufacturing process of protein-based therapeutics, but it may result in an undesired loss of biological activity. Many variables come into play during freezing that could adversely affect protein stability, creating a complex landscape of interrelated effects. In this article we show how mathematical models can guide the selection of the optimal freezing protocol, focusing on protein stability. Two opposite scenarios are identified, suggesting that the ice-water interface is the dominant cause of denaturation for proteins with high bulk stability, while the duration of the freezing process itself is the key parameter to be controlled for proteins that are susceptible to cold denaturation. Experimental data for lactate dehydrogenase and myoglobin as model proteins support the model results.

📌Anyone clicking on the following link before February 15, 2020, will be taken directly to the final version of our article on ScienceDirect, which they are welcome to read or download. No sign-up, registration or fees are required.

https://authors.elsevier.com/a/1aIVS55Jf1ZLc

“Congrats to all co-authors From our research group a new "Considerations on Protein Stability during Freezing and its Impact on the Freeze Drying Cycle: A Design Space Approach" https://t.co/gVhwBBReRp (QbD)”

06/12/2019

Come ben si sa, il giorno della discussione della tesi è il traguardo di un lungo percorso... e Giulia, Paolo, Livio e Gabriele sono prontissimi per l'Aula Magna 👨‍🎓
"Nessun limite oltre il cielo (Miguel De Cervantes)"
con Giulia Giulia Bucca; Livio Marenco ; Paolo Giorsello ; Gabriele Ruggiero 👏

28/11/2019

This brings together leading practitioners in the freeze-drying community to address recent progressin the manufacturing of lyophilized products.
📌I would like to thanks all the contributors, Andrea Arsiccio, Ivonne Seifert, Wolfgang Friess, Domenico Colucci, Alberto J. Ferrer-Riquelme, José Manuel Prats Montalbán, Geoff Smith, Yowwares Jeeraruangrattana, T.N. Thompson, Luigi C. Capozzi, Jos Corver. (✍️RP)
https://www.crcpress.com/Freeze-Drying-of-Pharmaceutical-Products/Fissore-Pisano-Barresi/p/book/9780367076801

Freeze Drying of Pharmaceutical Products provides an overview of the most recent and cutting-edge developments and technologies in the field, focusing on formulation developments and process monitoring and considering new technologies for process development. This book contains case studies from fre

14/11/2019

Congrats Fiora Artusio👏👏👏

“Congrats👏 to student Fiora Artusio👩‍🎓 for her excellent talk on at at ”

11/11/2019

Francesca Susa👨‍🎓ha partecipato all'evento .PoliTo
Il è stato promosso dal Politecnico di Torino per festeggiare i 160 anni.
https://www.festivaltecnologia.it/ è nato per esplorare la relazione tra tecnologia e società con un approccio umanistico e democratico.

23/10/2019

Andrea ... PhD Thesis Defense ..

17/10/2019

Dario Casà👨‍🎓
Yuri Kitamukai👨‍🎓
Lorenzo La Cortiglia👨‍🎓
" Tra vent’anni non sarete delusi delle cose che avete fatto ma da quelle che non avete fatto. Allora levate l’ancora, abbandonate i porti sicuri, catturate il vento nelle vostre vele. Esplorate, sognate, scoprite. - Mark Twain"

11/10/2019

"L'importante non è quello che trovi alla fine di una corsa... L'importante è quello che provi mentre corri." (prof. Martinelli)
Dario Casà
Yuri Kitamukai
Lorenzo La Cortiglia

01/10/2019

https://twitter.com/MolE_polito/status/1178991874826264576?s=20

26/08/2019

Congrats to Andrea who received the Timasheff award at the Colorado Protein Stability Conference 2019