13/05/2026
We are pleased to announce that on Friday, May 15, at 15:45 there will be a lecture led by a representative of Genomed, Piotr Małysa, titled "NGS sequencing in microbiome analysis - comparison of available sequencing approaches using the example of V3-V4 16S rDNA metabarcoding service at Genomed S.A."
Below is the abstract of the lecture:
Next-generation sequencing (NGS) currently represents one of the key tools used for the analysis of microbial diversity. These methods are widely applied in studies of the microbiome of various environments. An important step in experiment planning is the appropriate selection of a sequencing strategy, depending on the type of tested samples.
Genomed S.A. provides NGS services based on technologies developed by Element Biosciences (AVITI platform), Illumina (NovaSeq and MiSeq platforms), as well as long-read sequencing technologies - Oxford Nanopore Technologies and Pacific Biosciences. Each of these approaches has different technical parameters, costs and limitations.
One of the most commonly used, cost-effective, and sensitive approaches for microbiome studies is metabarcoding based on sequencing of the V3-V4 hypervariable regions of the 16S rDNA gene. At Genomed S.A., this service is performed on the AVITI platform in PE300 mode. Compared with analogous analyses performed on Illumina platforms, the AVITI system enables the generation of higher-quality sequencing data at a lower service cost while maintaining the standard output format (.fastq files).
The application of long-read sequencing technologies may further improve taxonomic resolution in microbiome analyses. However, these approaches are associated with higher costs and specific requirements regarding the quantity and quality of DNA extracted from the analyzed samples.
Genomed.pl
Presenter: Piotr Małysa