26/10/2023
Part one
我們的螢光蛋白量測硬體靈感來自於COVID-19快速檢測試劑盒，這些試劑盒因其便攜性、使用者友好性、即時性、準確性、易用性和價格親民而廣受歡迎。為了應對在人體內檢測早期炎症反應的挑戰，我們提出了一種炎症快速檢測試劑盒的概念，並依照專家意見和反饋制定了以下設計原則：便攜性、提供即時且精確的結果、使用者友好、易於取得且價格實惠、內建感應和記錄功能。
在第1版本中，我們的設備採用了10cm的正方形尺寸，以容納比色皿。外部設計設有一個鉸鏈上蓋，以在測量過程中創造完全的黑暗環境。這種設計消除了需要額外組件如螺絲的需求。為了測量光通量（流明），我們使用光敏電阻器和可變電阻，從而簡化了整個設備。
數據分析方面，第1版本的目標是檢查總光通量（包括激發光和散射光的組合）與純激發光光通量的比率，期望較高的比率能提供更好的結果。然而，第1版本的實驗數據並不足夠可靠，因為無法明確區分激發光強度的變化和散射光的影響。這導致了複雜的數據處理，使得設計變得不切實際。
Our fluorescent protein measurement hardware was inspired by COVID-19 rapid test kits, which have gained acclaim for their portability, user-friendliness, real-time results, accuracy, ease of use, and affordability. Addressing the challenges of detecting early inflammatory responses in the human body, we envisioned an inflammation rapid test kit. Guided by expert insights and feedback, we formulated the following design principles: portability, instant and precise results, user-friendliness, accessibility, affordability, and embedded sensing and recording.
In the first version, our device featured a 10cm square design to accommodate a cuvette. The external design included a hinged cover to create complete darkness during measurements, eliminating the need for additional components like screws. To measure light flux (lumens), we used a light-dependent resistor (LDR) and a variable resistor, simplifying the device.
Regarding data analysis, the primary goal of the first version was to examine the ratio of total light flux (comprising both excitation and scattered light) to pure excitation light flux, with higher ratios expected to yield better results. However, the experimental data from the first version was not reliable enough, as it was challenging to differentiate changes in excitation light intensity from the influence of scattered light. This complexity in data processing rendered the design impractical.
