10/09/2026
Less than 5 percent. That's how much of the corneal endothelium standard specular microscopy can capture.
This single layer of cells keeps the cornea clear. Clinicians assess it in Fuchs dystrophy, before cataract surgery, and after corneal transplantation. Yet the periphery remains the hardest part to image.
Ved Shivade, MS, and his team at NYU Grossman School of Medicine, in partnership with Case Western Reserve University, built Tessera, custom software that takes video from a widefield microscope and stitches the frames into one large, detailed mosaic in real time.
Technicians can watch the image build, spot any gaps, and recapture them before the session ends. In early results, Tessera mosaics covered up to 75 percent of the endothelium—137 to 254 times more area than a single frame. One mosaic mapped 62,595 cells. That's the largest cell count from a single endothelial image reported to date.
A wider view could help researchers track corneal health, disease progression, and responses to emerging therapies over time.
Ved Shivade, MS, explains how it works. Watch.